Fig. 8. Effect of PI3K inhibition on SFICM. (A) Cells were submitted to a 1.8 Pa shear stress in the presence of 34 µM LY294002 and monitored for 10 minutes under dark field illumination at a 2.5x magnification. A set of representative cell tracks is shown superimposed over the first image recorded. These data correspond to Movie 4. Each track starts on the cell near the corresponding number and ends up at the free end of the line. Scale bar, 100 µm. (B) Cells were submitted to a 1.8 Pa shear stress in the presence of the indicated LY294002 concentration and monitored for 10 minutes as in (A). The orthogonal projections of the average translational cell velocity over 10 minutes (<v_x> and (<v_y>) were determined using Eqn 2 and plotted as a function of LY294002 concentration. (C) Distributions of instant cell velocity modulus in the presence (gray bars) or the absence (white bars) of 34 µM LY294002. Applied shear stress is 1.8 Pa. Data are from Fig. 2A and Fig. 8A. (D) Angular distributions of cell instant velocities with respect to the flow axis in the presence (gray bars) or the absence (white bars) of 34 µM LY294002. The histogram of the directions of cell movement is represented as in Fig. 3C. Applied shear stress is 1.8 Pa. Data are from Fig. 2A and Fig. 8A.

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