Fig. 5. Co-localization of 6ß4 and D6.1A in BSp73AS-db cells. BSp73AS-db cells were seeded on recombinant Ln5. After 48 hours cells were stained with D6.1 or anti-CD9 plus FITC-labeled anti-mIgG and were counterstained with TxR-labeled B5.5 or were stained with B5.5 plus FITC-labeled anti-mIgG and were counterstained with TxR-labeled D6.1. As described in Fig. 4,Fig. 4, binding of the second, dye-labeled antibody to anti-mouse IgG was prevented by the addition of an excess of mouse IgG. Red and green fluorescence and the digital overlay are shown. Whereas in BSp73AS-db cells D6.1A and 6ß4 mostly co-localized irrespective of whether D6.1A or 6ß4 was cross-linked via the secondary antibody, CD9 hardly co-localized with 6ß4. Scale bar: 10 µm.

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