Fig. 5. Substitution of polar and aromatic residues in the TMD impairs formation of disulfide-linked hexamers. (A) ERGIC-53 constructs were probed for disulfide-linked oligomer formation as described in Fig. 2 (fluorogram). The constructs contained wild-type TMD (wt TMD) or the triple substitution F₄₈₄L/Q₄₈₈L/Y₄₉₈L (mt TMD, Fig. 4). (B) Quantification of fluorogram shown in A. Triangles, constructs with wt TMD; circles, constructs with mt TMD; filled symbols, dimers; empty symbols, hexamers. MWM, molecular weight markers. The result of a representative example is shown.

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