Fig. 1. Saturation of tGolgin-1 C312 localization in HeLa cells. HeLa cells transiently transfected with plasmid expressing HA-tagged C312 were analyzed 2 days later by IFM with anti-HA and RRX-conjugated secondary antibodies. (a-c) Examples of cells with different expression levels of C312 classified as levels I, II and III as indicated. (d) Comparison of semiquantitative total cell fluorescence levels with phenotypic classification. Total fluorescence from individual cells (in arbitrary units) was measured using OpenLab software as indicated in Materials and methods, and plotted on the y axis. Each dot represents the measurement from a single cell; the bar represents the median expression level from all cells.

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