Fig. 8. GRIP domain overexpression disrupts Golgi morphology. HeLa cells transfected with expression vectors for Tac and for (A) C312(Y2187A) or (B,C) C312 were sorted for Tac cell surface staining, exposed to HRP for 15-30 minutes at 37°C, and then fixed and embedded in epon for conventional EM analyses. Note the flattened stacks of Golgi complex (Gc) cisternae in A and their absence in B and C. m, mitochondrion; n, nucleus. In C, stars are placed next to multivesicular bodies, which were abnormally abundant in most profiles from C312-transfected cells. Bars, 0.1 µm.

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