Fig. 2. ß-Actin and ADF mRNAs are detected in axons by in situ hybridization with species-specific probes. Fixed neurons were hybridized with probes for the whole ß-actin sequence (A,B), the ß-actin 3' UTR (C,D) and the ADF partial sequence (E,F). Bright, punctate signals were detected in branch points, varicosities and growth cones, and also along the axonal shaft (Tables 1, 2). Neither cells treated with RNase prior to antisense probe hybridization (G,H) nor cells hybridized with sense probe (I,J) showed any signal. The controls shown were carried out with the full length ß-actin probe; separate control experiments for the ß-actin 3' UTR and ADF probes gave identical results. Scale bar, 20 µm.

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