Fig. 4. Cycloheximide-sensitive axonal translation produces a complex population of proteins. (A) SDS-PAGE and autoradiography of newly synthesized proteins in the axonal halo vs entire ganglion. Lanes 1,3,5 contain protein from 17 axonal halos; lanes 2,4,6 contain protein from 0.25 whole ganglia. Protein synthesis in whole ganglia and in axon halos (lanes 1,2) was eliminated by treatment with cycloheximide (lanes 3,4) but not with chloramphenicol (lanes 5,6). (B) The protein composition of axonal halos is different from that of entire ganglia (lanes1,2, enlarged area at right). The arrow at left indicates a protein whose synthesis is highly enriched in the axon and the arrows at right indicate two proteins whose synthesis is highly enriched in the cell bodies. (C) Close examination using 4-15% gradient SDS-PAGE and autoradiography identifies the molecular weight of prominent proteins enriched in the axon (arrows at left) or cell bodies (arrowheads at right). 40 axonal halos (lane 1) and 1 ganglion (lane 2) were loaded.

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