Fig. 3. Models of dynein-dependent nuclear migration and organelle transport. (A) Dynein-dependent nuclear migration. Microtubules emanate from the SPB, with their plus ends (+) distal to it. Immotile dynein is recruited to the microtubule distal end together with dynactin and LIS1 by CLIP-170. Dynein, dynactin and LIS1 are delivered to the dynein-anchoring factor (Num1 or a Num1-delated factor, ApsA) on the cell cortex by microtubule elongation. The anchoring factor anchors dynein, dynactin and LIS1, and activates dynein motility. The motile dynein moves on the microtubule toward the minus end, driving nuclear migration toward the cortex. (B) Organelle transport. Dynein, dynactin and LIS1 are delivered to the dynein-anchoring factor on the organelle surface by microtubule elongation, as in A. The anchoring factor anchors and activates dynein. The activated dynein moves on the microtubule, transporting the organelle. The plus (+) and minus (–) ends of the microtubule are indicated. Black arrows indicate microtubule elongation, and red arrows indicate dynein movement.

Return to article