Fig. 3. Ct-eIF4H is present in polysomes and can be UV-cross-linked to cytoplasmic poly(A+) RNA. (A) Polysomes from C. tentans tissue culture cells were pelleted by centrifugation. Ct-eIF4H, detected by western blotting, was present in the pelleted polysomes (lane 1). The polysomes were resuspended, treated with 0.5 M KCl and repelleted. Ct-eIF4H was then released from the polysomes (lane 2) and found in the supernatant (lane 3). (B) C. tentans tissue culture cells were UV-irradiated, and poly(A+) RNA was isolated from cytoplasmic extracts. After elution of the RNA from the oligo (dT) cellulose and RNase treatment, the co-purified proteins were analyzed by western blotting. Ct-eIF4H (lane 1) and hrp36 (lane 3) co-purifies with poly(A+) RNA after UV-cross-linking but not when UV-cross-linking was left out (lanes 2 and 4, respectively).

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