Fig. 8. Electron micrographs of anti-Ct-eIF4H antibody labeling in the interchromatin and in the cytoplasmic perinuclear region in C. tentans salivary gland cells. A schematic drawing of each micrograph is shown to the right to simplify interpretation. The nuclear membrane is represented by the dashed lines and the gaps indicate the positions of NPCs as shown in the schematic drawing of B. In each micrograph, the nucleus (Nuc) and cytoplasm (Cyt) are indicated. Gold particles are denoted by black dots. (A) BR mRNP particles (circles marked BR in the schematic drawing) in the interchromatin and docking at NPCs (arrow) were not labeled by the anti-Ct-eIF4H antibodies. (B) A Ct-eIF4H-specific gold particle associated with the cytoplasmic side of a NPC. This is also seen for the two gold particles in the cytoplasmic perinuclear region in A. (C,D) Ct-eIF4H-specific gold particles associated with electron-dense fibers extending from NPCs into the cytoplasmic perinuclear region. These fibers represent extended BR mRNP particles during passage through the NPCs. In the schematic drawing these fibers are marked BR. Bar, 200 nm.

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