Fig. 4. Expression of 5T4 antigen in differentiating ES cells is associated with the differentiation rate. (a) Expression of 5T4 antigen correlates with cell migration in differentiating ES cells. (i) MESC and (ii) 129 ES cells (10⁵ cells/3 cm dish) were grown for 0, 3 and 6 days in DMEMSR in the absence of LIF and viewed under phase contrast optics on an Olympus inverted microscope. Small arrows indicate undifferentiated ES cells and large arrows differentiated/migrating cells. (b) Expression of 5T4 correlates with the proliferation rate of differentiating ES cells. (i) MESC and (ii) 129 ES cells (10⁵ cells/3 cm dish) were grown in DMEMSR medium in the absence (squares) or presence (diamonds) of LIF (arrow indicates day of LIF removal) for 3 days and the number of viable cells determined by light microscopy of cells excluding Trypan Blue. Scale bar: 10 µm.

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