Fig. 6. Presence of 5T4 on ES cells is a measure of decreased pluripotency. 129 ES cells were cultured in (a) the presence or (b) absence of LIF for 6 days and (i) SSEA-1-positive cells isolated by FACS (boxed area). (ii) 5T4 expression of the SSEA-1-positive population was determined as described in the legend to Fig. 1a. Pluripotency of the SSEA-1-positive cells was determined by chimera formation following injection of 15 cells into 3.5-day-old BL/6 blastocysts and subsequent implantation into pseudo-pregnant BDF-1 female mice.

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