Fig. 5. Bpag1e localizes predominantly to the nucleus in C2C12 myoblasts. (A-C) In cells double-labeled for Bpag1e (A) and paxillin (B), overlap in the patterns of staining was observed in the center of a few cells (arrows in insets; insets are enlargements of the center region from the larger cell). However, the only staining in most cells was light staining in the nuclei (arrowhead in A). (D,E) Bpag1e staining (D) and Hoechst staining (E) showing that most of the Bpag1e staining was limited to the nuclei (arrowheads in D and E). In several mitotic cells noted, Bpag1e staining was absent from condensed chromosomes (arrows in D and E). (F,G) Bpag1e staining (F) was also identified in the nuclei (G; arrowheads in F and G) using bullous pemphigoid patient serum [Bpag1e (10-71)]. Bars, 20 µm.

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