Fig. 2. Hypochondrodysplasia-like phenotype in growth plates of junB^–/– Ubi-junB mice. (A) Growth plate morphology of proximal tibiae from 4-, 8- and 16-week-old control (311K) and junB^–/– Ubi-junB (311R) littermates. Black lines indicate differences in the size of control and mutant growth plates. Sections were stained with Haematoxylin and Eosin. PC, proliferative chondrocytes; HC, hypertrophic chondrocytes. Magnification was 20x (4 weeks, 8 weeks) and 40x (16 weeks). (B) In situ detection of type-II (col-II), type-X collagen (col-X), alkaline phosphatase (alp) and vascular endothelial growth factor (vegf) mRNAs in growth plates of femur sections prepared from 4-week-old control (middle panel) and junB^–/– Ubi-junB (right panel) littermates. Hybridisation of control sections with sense probes served as control for specificity (left panel). Dark-field images were used to demonstrate vegf expression in hypertrophic chondrocytes. Sections were counterstained with Haematoxylin and Eosin; magnification was 20x.

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