Fig. 7. Deregulated expression of Cyclin A and p16^INK4a in junB^–/– Ubi-junB chondrocytes and osteoblasts. Reduced proliferation of growth plate chondrocytes and osteoblasts in junB^–/– Ubi-junB long bones was demonstrated by immunohistochemistry with a monoclonal anti-PCNA antibody (A-E). Cyclin A (F-J) and p16^INK4 (K-O) protein levels were determined by immunohistochemistry using polyclonal anti-Cyclin A or anti-p16^INK4a antibodies, respectively. Arrowheads indicate Cyclin A-positive chondrocytes and osteoblasts in control sections (G,I) and chondrocytes and osteoblasts with increased expression of p16^INK4 in mutant sections (M,O). PCNA-positive (E) and Cyclin A positive (J) haematopoietic cells were still present in junB^–/– Ubi-junB bone marrow (arrows). Control sections were incubated only with second antibody (A,F,K). Sections were counterstained with Haematoxylin; 40x magnification.

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