Fig. 5. DRONC and DRICE caspases are involved in selD^ptuf apoptosis. (A) Overexpression of DRONC caspase under the control of GMR-GAL4 driver gives rise to slightly rough and mottled eyes as a result of ectopic cell death. white patches result from the ablation of pigment cells. (B,C) Reduction of one dose of selD or catalase, respectively, enhanced the mottled eye phenotype. Wider areas of white tissue were observed indicating that an increase in ROS levels enhances DRONC apoptotic activity. (D) Combined reduction of selD and catalase strongly enhanced DRONC overexpression phenotype and few pigmented cells were observed. (E) Active DRICE (red) in a selD^ptuf homozygous mutant imaginal disc. The cell surface was outlined using anti-Fasciclin III antibody (green). (F) Active DRICE in selD^ptuf clones (dark area lacking ß-galactosidase labeling, green) in the eye disc. Consistent with the finding of non autonomous apoptosis and ROS, active DRICE was found as well outside the clone. (G) Overexpression of Dmp53 triggers apoptosis through DRICE caspase as shown by the presence of active DRICE (red) in gl-Dmp53 discs. In E, F and G lower panels are merged images.

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