JCS00792 Supplemental Data

Files in this Data Supplement:

• Supplemental Figure 1 -

  Fig. S1. Differential distribution of adhesion proteins in focal complexes and focal adhesions at the leading edge of migrating endothelial cells. Cells migrating into an in vitro ?wound? were fixed and immunolabeled for phosphotyrosine (left column) and different focal adhesions proteins (middle column). Analysis of the differential distribution of the different proteins relative to phosphotyrosine (used here as a marker for both focal adhesions and focal complexes) was assisted by pixel-to-pixel calculation of the intensity ratio. Ratio images are presented in the right column. Scale bar: 5 mm.

• Supplemental Figure 2 -

  Fig. S2. Absence of zyxin from focal complexes in a variety of cells, including fibroblasts (REF52), chondrocytes (RCJ) and melanoma (B16) cells. The cells were fixed 2 hours after plating and double stained for phosphotyrosine and zyxin. Scale bar: 5 mm.