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Fig. 4. Shift of TJ proteins from soluble to insoluble phase. Confluent CCR2+/+ and CCR2–/– mBMEC were subjected to 100 nM MCP-1 for 15 minutes or 2 hours. Triton X-100-soluble and Triton X-100-insoluble fractions were collected. Immunoblots of those fractions were then probed with anti-occludin, anti-claudin-5, anti-ZO-1 and anti-ZO-2 antibodies. (B) Immunoblots were analyzed and quantified with NIH Image software. Data represent means±s.e.m. of three independent experiments.





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