Fig. 8. Effect of inhibition of RhoA and Rho kinase on MCP-1-induced alterations in actin and TJ proteins. Confluent mBMEC were pretreated for 30 minutes with 10 µM Y27632, or 18 hours with 5 µg/ml C3 exoenzyme, or transiently transfected with T19NRho and then were exposed to 100 nM MCP-1 for 2 hours. The cells were then fixed and processed for immunocytochemistry using anti-ZO-1, -ZO-2, -occudin, and claudin-5 antibodies and Alexa 488 Phalloidin for F-actin. Arrows indicate organization of actin and TJ proteins in presented experimental groups. Scale bar: 200 µm.

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