Fig. 4. Histologic analysis of LD-induced injury in WT and K8-null mouse gallbladder and liver. K8 mice (–/–, +/+) were fed a normal or lithogenic diet for 5 weeks. Gallbladders and livers were resected, fixed, paraffin embedded, sectioned, and then stained with Hematoxylin and Eosin. (a-d) Gallbladder sections from mice fed a normal (a,b) or a lithogenic (c,d) diet. E, single layer of cuboidal epithelial cells; M, muscularis; I, small foci of mononuclear inflammatory cells. (e-h) Liver sections from mice fed a normal (e,f) or a lithogenic (g,h) diet. (f) A focal area of inflammation (arrow) and a small area of hemorrhage (arrowhead). (g) A small area of inflammation and necrosis (arrow). Panel h shows a markedly vacuolated cytoplasm with displacement of the nuclei to the cell periphery, Kupffer cell nodule (arrow) and hepatocyte erythrophagocytosis (arrowhead). The histologic findings of the liver and gallbladder of K8^+/– mice that were fed a normal diet or LD (not shown) were very similar to those shown for the corresponding K8^+/+ mice. Scale bar (in a): 100 µm for a-d and 200 µm for e-h.

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