JCS00727 Movies

Files in this Data Supplement:

• Movie 1 -

  Movie 1. TeNT H_C-Alexa488 retrograde transport along the axon of an untreated rat MN (control for the Lat B and Vin treatments described in Figs 3 and 4). The cell body is located at the bottom. Frames were taken every 5 seconds. Shown here is a movie consisting of 31 frames played at 5 frames/second. The image is 15 mm x 43 mm. See also Fig. 3A.

• Movie 2 -

  Movie 2. Effect of F-actin depolymerisation on TeNT H_C retrograde transport. Treatment of rat MNs with 0.5 mM Lat B for 20 minutes causes the majority of TeNT H_C endocytic structures to stop or oscillate. The cell body is located at the bottom. Frames were taken every 5 seconds. Shown here is a movie consisting of 31 frames played at 5 frames/second. The image is 11 mm x 38 mm. See also Fig. 3B.

• Movie 3 -

  Movie 3. Effect of MT depolymerisation on TeNT H_C retrograde transport. Treatment of rat MNs with 5 mM Vin for 50 minutes causes the majority of TeNT H_C carriers to stop or oscillate. The cell body is located at the bottom. Frames were taken every 5 seconds. Shown here is a movie consisting of 32 frames played at 5 frames/second. The image is 9 mm x 35 mm. See also Fig. 4A.

• Movie 4 -

  Movie 4. TeNT H_C retrograde transport along a wild type (left) and a homozygous dilute lethal (right) MN axon. Both cell bodies are located at the bottom. Frames were taken every 5 seconds. Shown here is a movie consisting of 45 frames played at 5 frames/second. Each image is 7 mm x 35 mm. See also Fig. 6D and E.