Fig. 3. Retrograde transport of TeNT H_C depends on F-actin. A scheme of the experiment is shown at the top. MNs were treated with DMSO (Ctrl) (A) or with 0.5 µM Lat B (B). Intervals between frames are 5 seconds. Cell bodies are located at the bottom. (A) Control neurons display retrograde transport (arrowhead, arrow, asterisk). See video 1. (B) Lat B causes the majority of carriers to stop or oscillate (arrowhead). See video 2. (C) Quantitative analysis of TeNT H_C transport after F-actin disruption. Carriers were classified as stationary/oscillatory when the extent of the movement did not lead to any significant progression (<0.2 µm). Results are expressed as a percentage of the total carriers observed (control = 437, Lat B = 315). Bars represent the s.d. of four independent experiments (P=0.01). (D) Treatment with Lat B does not affect MTs visualized by an anti-ß-tubulin antibody. F-actin staining in MNs (E) disappears after Lat B treatment (F). Bars, 5 µm (A,B and E,F) and 10 µm (D).

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