Fig. 3. Morphogenetic perturbations induced by the Ral-GDS binding, Raf non-binding, Ras 12V37G. (A) Phenotypic effects at the blastula (stage 8) and neural plate (stage 14) stages resulting from Ras mutant mRNA injections. mRNAs encoding Ras 12V35S and 12V37G (500 pg/blastomere) were injected into the animal pole of each blastomere in two-cell embryos. The arrow indicates patches of abnormal cells in embryos injected with Ras 12V37G. Embryos co-injected with Ras 12V37G and XralB S28N were developmentally arrested during gastrulation but did not display necrotic cells. (B) Analysis of the cortical actin cytoskeleton in embryos injected with either 12V35S or 12V37G, and the rescue effect of XralB S28N on Ras 12V37G. Embryos were injected with Ras 12V35S or Ras 12V37G mRNA (500 pg/blastomere), or co-injected with Ras 12V37G (500 pg/blastomere) and XralB S28N (3 ng/blastomere), respectively. The arrowhead shows the reconstituted cortical actin cytoskeleton in embryos co-injected with Ras 12V37G and RalB S28N mRNAs. The actin cytoskeleton of animal caps was analysed at the MBT stage. Scale bars: 50 µm; confocal optical sections are 1 µm.

Return to article