Fig. 3. Effect of heparitinase on the localization of III_1c in the cell layer. Human fibroblasts were seeded onto glass coverslips and grown as previously described. Wells were then incubated with or without 0.01 U/ml heparitinase I for 2 hours followed by the addition of 20 µM III_1c or III₁₃ for 2 additional hours. Cells were then washed, fixed, permeabilized and dual stained for fibronectin (FN) using a polyclonal antibody and the recombinant fragments using a monoclonal antibody against the His-tag (HIS) as indicated. Fields shown represent one experiment repeated three times.

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