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Fig. 5. TgSODB2 is imported into the mitochondrion. A western blot of lysate from parasites stably expressing TgSODB21-287/GFP shows two bands (grey arrows) in addition to the endogenous TgSODB21-287 and TgSODB285-287 bands (black arrows) seen in 2F lysate: a faint band at about 60 kDa corresponding in size to TgSODB21-287/GFP and a much more abundant product at about 50 kDa corresponding to TgSODB285-287/GFP. m{alpha}rSODB2 recognized all four bands, whereas {alpha}GFP recognized only the 50 and 60 kDa, GFP-containing products. HFF cell lysate is included as a negative control.





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