Fig. 1. Efficient depletion of Topo II in Drosophila S2 cells using RNAi. (A) Immunoblots: (upper) Topo II levels begin to fall by 48 hours after the addition of dsRNA and become undetectable by 72 hours (1x10⁶ cells loaded per lane); (lower) loading control (anti-tubulin). –, control RNAi; +, Topo II RNAi treated. (B) The anti-Topo II antibody used can detect Topo II in 5x10⁴ cells, but not 1x10⁴ cells. (C-F) Immunofluorescence analysis showing Topo II depletion at the 72nd hour after treatment; (C,E) controls, (D,F) treated cells, (C,D) prometaphase, (E,F) anaphase. In all merged images, DAPI is blue, Cid is red and Topo II is green. Scale bar: 5 µm.

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