Fig. 8. Anaphase in Topo II-depleted cells is characterised by the presence of massive chromatin bridges, however sister centromeres usually manage to disjoin and move towards the spindle poles. (A) Control RNAi-treated normal anaphase cell. (B) Topo II RNAi-treated anaphase cell with the bulk of the chromatin stretched out between the separating kinetochores. (A-B) DAPI staining for DNA; (A'-B') anti-tubulin shows the mitotic spindle; (A"-B") staining for Cid/CENP-A shows the position of kinetochores; (A'"-B'") merged images (DAPI is blue, tubulin is red, Cid is green). Scale bar: 5 µm. (C) Statistical analysis of the lagging chromosome phenotype.

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