Fig. 5. Microtubules are required to retain dynein, but not dynactin, at pronuclear surfaces. (A) Dynein-intermediate-chain localization to the female pronucleus is no longer detected in bovine zygotes following nocodazole treatment (left). Microtubules (MTs), observed around both female (F) and male (M) pronuclei in controls, are not seen in nocodazole-treated zygotes. Dynactin p150^Glued, however, localizes to both pronuclei in nocodazole-treated bovine zygotes, even when microtubules are no longer detected (right). Scale bars, 10 µm. (B) Quantification of the effects of nocodazole treatment on dynein and dynactin association with pronuclei. Results represent the average ± s.d. of four sets of experiments, with a total of 512 zygotes examined for dynein staining; P<0.001 compared with controls (Student's t test). A total of 504 zygotes were examined for dynactin staining, with the differences not statistically significant compared with controls (Student's t test).

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