Fig. 3. Normal localization and expression patterns of caveolin-1 in the presence of the TFT mutant caveolin-3. (A) Myoblasts stably expressing the TFT mutation or vector alone (Control) were analyzed for localization of caveolin-1. Caveolin-1 localized almost exclusively to the plasma membrane and no differences between cell populations were observed. (B) The level of caveolin-1 and caveolin-3 expression was assessed by western blot analysis in myoblasts maintained in GM or undergoing differentiation in DM. Expression was analyzed 1, 2 and 3 days after the initiation of differentiation. In control cells and in cells expressing the TFT mutation, caveolin-1 was highly expressed in myoblasts and declined during myogenic differentiation. Expression of caveolin-3 increased with differentiation in control cells but was expressed at high levels throughout differentiation in cells constitutively expressing the TFT mutation. Actin was used as the loading control in all experiments.

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