Fig. 5. Reduced albumin uptake in myotubes expressing the TFT mutant caveolin-3. (A) Control myotubes and myotubes expressing the TFT mutation were incubated with Texas Red-conjugated albumin (20 µg/ml) for 30 minutes and then analyzed by fluorescence microscopy for cellular uptake of Texas Red. In control myotubes, Texas Red was observed in foci throughout the cytosol (arrows) and surrounding the nucleus (nuc). In contrast, only occasional Texas Red-positive foci were observed in the cytosol of myotubes expressing the caveolin-3 mutant. (B) Control myotubes and myotubes expressing the TFT mutation were incubated with Texas Red-conjugated albumin as described above, and total cell lysates were analyzed by western blotting using an antibody against Texas Red. The levels of Texas Red were substantially lower in myotubes expressing mutant caveolin-3 compared with control myotubes. Actin was used as the loading control.

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