Fig. 8. The TFT mutant caveolin-3 causes an increased incidence of apoptosis in myotubes. Control myotubes and myotubes expressing the TFT mutation were stained at day 1, day 3 and day 6 of differentiation using the TUNEL assay. The percentages of myotubes containing TUNEL-positive nuclei were determined. At least four non-overlapping fields were counted for each culture, and four separate cultures for each cell population were analyzed. At day 3 and day 6 of differentiation, there were significantly more apoptotic TFT-expressing myotubes than apoptotic control myotubes. Data are presented as mean±s.d. At each time point, one-way analysis of variance was used to compare the data for each mutant to the control data (^*P<0.05, ^**P<0.005).

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