Fig. 3. The a1 isoform of the V-ATPase a subunit is concentrated in nerve terminals. The a1-subunit distribution in different tissues (liver, kidney, brain and electric lobe) was studied by western blotting of tissue aliquots containing similar amounts of the V-ATPase c subunit (c-V-ATPase) and compared with the distribution in the same samples of the nerve terminal SNARE proteins VAMP-2 and syntaxin-1. The distribution of these antigens in the somatodendritic (electric lobe) and nerve terminal (synaptosome) compartments of T. marmorata electromotoneurons was compared. The protein amount in each sample is indicated (µg protein). SV, purified synaptic vesicles.

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