Fig. 4. Localisation of Topo II to mitotic chromatin after depletion of DmSMC4. (A) In control cells DmSMC4 and Topo II partially co-localise to an axial structure of sister chromatids during both prometaphase or anaphase. Some accumulation of Topo II is also observed at the centromeres. Merged images show DNA (red) and Topo II (green). (B) Detailed analysis of distribution of DmSMC4 and Topo II in prometaphase chromosomes. The merged image shows DmSMC4 (green) and Topo II (red). Higher magnification view of selected regions (a and b) indicated in the merged image, show that Topo II and DmSMC4 localise mostly as an alternating string pattern with some regions in which both proteins are found. (C) After depletion of DmSMC4, Topo II does not show a defined localisation to the axial structure within sister chromatids but becomes diffusely distributed all over condensed chromatin and in chromatin bridges. Merged images show DNA (red) and Topo II (green). Scale bars: 5 µm.

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