Fig. 3. The importin /ß complex prevents the recruitment of Aurora A to microtubules and its activation. (A) Taxol stabilized microtubules (MT) were incubated with mitotic HeLa cell extract in the presence or absence of GST-RanL43E and the importin /ß complex then re-isolated by centrifugation and the pellet fractions analyzed by SDS-PAGE and immunoblotting to detect TPX2 and Aurora A. (B) Taxol stabilized microtubules were incubated with mitotic HeLa cell extract in the presence or absence of GST-RanL43E or the importin /ß complex. After 30 minutes the extracts were analyzed by SDS-PAGE and immunoblotting to detect Aurora A phosphorylated on amino acid residue threonine 288 (P-Aurora A), then stripped and re-probed to detect all classes of Aurora A.

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