Fig. 3. Localization of early biosynthetic proteins. HeLa cells were transfected with mycp25SS alone and then incubated with anti-myc antibodies as in Fig. 2B, or triply cotransfected with p25SS, p23 and HA-p24 and then incubated with anti-HA antibodies. The distribution of antibody-tagged myc-p25SS or HA-p24 was then compared with those of the indicated proteins by indirect immunofluorescence. Cells transfected only with mycp25SS are shown in the panels that also illustrate the distribution of calnexin, ERGIC53, GRASP55 and p115, while triply co-transfected cells are shown in the panels that also illustrate the distribution of giantin and COP-I. The central column shows merged micrographs, color-coded as indicated. Scale bars: 10 µm.

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