Fig. 1. Localisation of ECFP-KV4.2 within the secretory pathway. HeLa cells were transfected with plasmid encoding ECFP-Kv4.2. After fixation the cells were immunostained with anti-PDI (A-C), anti-ERGIC-53 (D-F), anti-ß-COP (G-I) or anti--adaptin (J-L) visualised with Texas Redstreptavidin and imaged by laser scanning confocal microscopy. Black and white images are shown in each case for ECFP-Kv4.2 and antibody staining as indicated in the overlaid images shown in colour (Kv4.2 in green and antibody staining in red with overlap seen in yellow). Scale bar: 10 µm.

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