Fig. 10. Comparison of the localisation of KChIP1 and markers for the transitional ER and ERGIC compartment. HeLa cells were transfected to express KChIP1-EYFP or KChIP1(1-11)-EYFP, fixed, immunostained with the indicated antibodies visualised with Texas Red-streptavidin and imaged by laser scanning confocal microscopy. HeLa cells in the bottom row were transfected with KChIP1(1-11)EYFP and ECFP-Kv4.2. Black and white images are shown in each case as indicated with overlaid images shown in colour with KCHIPEYFP or KChIP1(1-11)-EYFP in green and antibody staining or ECFP-Kv4.2 in red and overlap seen in yellow. Scale bar: 10 µm.

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