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Fig. 2. Effect of KChIP1 on the localisation of ECFP-Kv4.2. HeLa cells were transfected with plasmids encoding ECFP-Kv4.2 and EYFP-Mem together (A-C) or in a triple transfection in combination with pcDNA3-KChIP1 (D-F). The localisation of ECFP-Kv4.2 and EYFP-Mem are shown individually and in a combined colour image (overlay; ECFP-Kv4.2 in green, EYFPMem in red) with co-localisation shown in yellow. Triple transfection resulted in translocation of ECFP-Kv4.2 to regions of the plasma membrane. Scale bar: 10 µm.





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