Fig. 5. Localisation of KChIP1 requires myristoylation and a minimal myristoylation motif is sufficient. HeLa cells were transfected to express KChIP1-EYFP (A,C), KChIP1(G2A)-EYFP (B,D), KChIP1(1-11)-EYFP (E,F) or KChIP1-ECFP and KChIP1(1-11)-EYFP (G,H) and examined after 3 days (A-D, F-G) or 1 day (E). The cells in C and D were fixed and examined after permeabilisation with 1 µM digitonin for 15 minutes in the absence of added Ca²⁺. The images in G and H are from the same co-transfected cell after 3 days. Scale bar: 10 µm.

Return to article