Fig. 7. Localisation of NCS-1, KChIP1 and Kv4.2 in PC12 cells. PC12 cells were transfected to express NCS-1-ECFP (A), hippocalcin(1-14)-EGFP (B), KChIP1-EYFP (C), KChIP1(1-11)EYFP (D), ECFP-Kv4.2 (E) or ECFP-Kv4.2 along with KChIP1-EYFP (F). KChIP1-EYFP and KChIP1(1-11)-EYFP showed more punctate intracellular staining than NCS-1-ECFP and hippocalcin(1-14)-EGFP and the latter constructs that were also localised to the plasma membrane. For the cells co-expressing ECFP-Kv4.2 and KChIP1-EYFP only the image for ECFP-Kv4.2 is shown in F but essentially complete overlap of the two constructs was observed. Scale bar: 10 µm.

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