Fig. 8. Effect of mutations within the myristoylation motifs of hippocalcin and KChIP1 on intracellular targeting. (Top) N-terminal sequences of hippocalcin and KChIP1 showing the mutations introduced and the positions of the conserved basic residues in NCS-1 and hippocalcin (arrows). (A-H) Localisation of minimal myristoylation sequences with the indicated mutations is shown after expression of EGFP (hippocalcin) or EYFP (KChIP1) fusion proteins in HeLa cells. The localisation of KChIP1(A3K,G6S, T7K, S9R)-EYFP was examined in cells co-transfected to also express NCS-1-CFP and the localisation of each fusion protein is shown individually and in an overlaid colour image where co-localisation is seen in yellow. Scale bar: 10 µm.

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