Fig. 9. Quantification of the effect of KChIP1 on ECFP-Kv4.2 traffic to the plasma membrane and requirement for correct targeting of KChIP1. (A) HeLa cells were transfected to express ECFP-Kv4.2 alone or in combination with KChIP1-EYFP or KChIP1(G2A)EYFP as indicated. ECFP-Kv4.2 fluorescence was imaged and quantified by drawing around regions of interest on the outside and the inside of the plasma membrane to allow determination of the percentage of total fluorescence at the plasma membrane. (B) Mean data±s.e.m. derived from 25 cells expressing ECFP-Kv4.2 alone and 47 and 24 co-expressing KChIP1-EYFP or KChIP1(G2A)-EYFP, respectively. (C) HeLa cells transfected to co-express KChIP1(A3K,G6S,T7K,S9R)-EYFP (KChIPmut) and ECFP-Kv4.2 did not show cell surface expression of Kv4.2. The overlaid colour image shows Kv4.2 in red and KChIP1(A3K,G6S,T7K,S9R)-EYFP in green with co-localisation in yellow. Scale bar: 10 µm.

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