Fig. 4. Identification of ß-dystrobrevin binding site on kinesin. (A) Schematic representation of the Kif5A truncation clones. The positions of the amino acids are indicated. The ß-dystrobrevin-binding site on Kif5A was found to be between amino acids 804 and 934, and is indicated in black. (B) Pull-down assay: each [³⁵S]Kif5A truncation protein was incubated with purified GST-ß-dystrobrevin pre-bound to glutathione-Sepharose beads. Radioactive proteins were separated by SDS-PAGE and detected by autoradiography. An aliquot of labeled starting material is shown on the left panel (input); the bound Kif5A truncated proteins are on the right (pull-down). Molecular masses of size markers are indicated on the left-hand side (in kDa).

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