Fig. 5. Direct interaction of ß-dystrobrevin and ubiquitous kinesin heavy chain. COS-7 cell lysate was incubated with GST-ß-dystrobrevin (GST-ß-DB) or GST-protein (GST), pre-bound to glutathione-Sepharose beads. After extensive washing, bound proteins were separated by SDS-PAGE and transferred to nitrocellulose. The blot was probed with polyclonal anti-uKHC antibody, and revealed by ECL. Molecular masses of size markers are indicated on the right-hand side (in kDa). WB, western blot.

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