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Fig. 3. Biglycan and decorin affect {alpha}-SMA. (A-D) Fibroblasts were plated (5000 cells/well) (A) without treatment or stimulated with (B) 10 ng/ml TGF-ß, (C) 10 µg/ml biglycan or (D) 10 µg/ml decorin for 24 hours. {alpha}-SMA was detected using a monoclonal mouse antibody against human {alpha}-SMA. Arrows indicate the protruding edge. (E) The protruding cell area stained for {alpha}-SMA and paxillin. Cells were stained with monoclonal mouse antibody against human {alpha}-SMA or paxillin, followed by Alexa Fluor® 584 goat anti-mouse IgG. (F,G) Western blot shows the total level of {alpha}-SMA in the cells after treatment. n=3.





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