Fig. 5. Biglycan and decorin induce activation of RhoA and Rac1. (A) After stimulation of human lung fibroblasts with 10 µg/ml biglycan and decorin, the amount of active, GTP-loaded RhoA and Rac1 was determined by GST-pull down assays with GST-C21 and GST-PAK-CD, respectively. (B) To confirm similar amounts of protein, the cell lysate was also subjected to western blotting for measuring the total amounts of RhoA and Rac1. (C) The intensity of the western blot of the GTP-loaded RhoA and Rac1 in relation to the intensity of the total amount of RhoA and Rac1, respectively. A-C show representative experiments after stimulation with biglycan and decorin. Values are given as means±s.e.m. for n=3-5; ^*significant differences of treatment compared to control.

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