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Fig. 6. (A) Western blot analysis on total protein extracts obtained from mice sacrificed 15 minutes, 1 hour or 3 hour after saline, SKF82958, pilocarpine or kainic-acid injection. 15 µg of proteins were separated on SDS/PAGE gels and revealed using anti-P.ERK, anti-panERK (ERK), anti-P.H3 (P), anti-Ac14.H3 (Ac14) and anti-P-Ac14.H3 (P-Ac14) antibodies. (B) Phosphorylation-acetylation of histone H3 on c-fos and MKP-1 chromatin. ChIP assays were performed on chromatin obtained from hippocampi of mice injected with 35 mg kg–1 kainic acid (GLU) or saline treated (–), using a preimmune serum (no Ab), anti-P.H3 (P), anti-Ac14.H3 (Ac14) or anti-P-Ac14.H3 (P-Ac14). The DNA recovered from the antibody-bound fraction and the DNA from the input chromatin were analysed by PCR with oligonucleotides specific for the indicated gene.





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