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Fig. 2. Dlg expression in E6 cell lines. (A) Western blot analysis of Dlg in cell extracts of E6 cell lines, Neo and HeLa cells. A decrease in total cell levels of Dlg correlates with expression of E6 with an active PDZ-binding motif. Position of markers of Mr (kDa) are as indicated. Actin levels control for loading. (B) Immunofluorescence microscopy of cells at high density stained with the anti-Dlg MAb 2D11. In Neo cells Dlg is recruited to the cell periphery at sites of intercellular contact. Peripheral levels of Dlg are significantly decreased in E6 cells expressing an active PDZ-binding domain (WT18E6 and Leu18E6), but are only partially reduced in cells expressing an E6 unable to degrade Dlg (Glu18E6). Bar, 20 µm.





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