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Fig. 3. Morphology of E6 cells. Cells seeded onto culture dishes at a density of 
5x105 cells/ml were allowed to adhere overnight before being photographed in a temperature-controlled stage chamber. a,a', Neo; b,b', Glu18E6; c,c', WT18E6; d,d', Leu18E6. (a-d) Light microscopy of cell cultures shows that cells expressing an active E6 PDZ-binding domain (WT18E6 and Leu18E6) were less likely to associate into organized colonies compared with control cells (Neo) or cells expressing an E6 with an inactive PDZ-binding motif (Glu18E6). Phase contrast microscopy (a'-d') of cells at higher magnification shows that E6 cells have undergone shape change and increased spreading. These effects were more prominent in WT18E6 and Leu18E6 cell lines. Bars, 100 µm (a-d) and 50 µm (a'-d').
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