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Fig. 1. Ezrin is expressed in the apical domain of ciliated airway epithelial cells in vivo. (A) Mouse lungs were immunostained with anti-ezrin (clone 3C12, green) and ß-tubulin-IV (red) antibodies to identify cilia. (B) Ezrin localization detected as in A highlighted by confocal microscopy with diffusion interference contrast. (C) Mouse lungs were immunostained with anti-ezrin (clone 3C12, green) and anti-Clara cell secretory protein (CCSP) antibody (red). Tissue sections (6 µm) were incubated with primary antibodies and detected using fluorescent-labeled secondary antibodies. Representative sections are shown. Bars 10 µm (A,C); 7 µm (B).





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